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Store lyophilized material at 4 F (20 C) or below Allow vial to reach room temperature before opening Protect from light, heat, and humidity Use sterile laboratory equipment during preparation Avoid repeated freezethaw cycles Label reconstituted samples with preparation date and concentration These practices support consistent experimental outcomes and reduce variability across assays

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The body is forced to switch to inefficient, anaerobic (oxygen-free) energy production, which generates lactic acid and further exacerbates systemic pain and fatigue

In general, pain is inversely correlated with prostaglandin levels